was performed by using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturers’ protocols. Briefly, cervical cancer cells were plated into 96-well plates or six-well plates. The cells were trans-fected with siRNA 1, siRNA 2, or negative control (1– 2μg) for 6–8h and then replaced with the fresh medium.
In this study, we aimed to assess common media additives that impede efficiency mediated by three chemical transfection agents: liposomal-based (Lipofectamine 2000), polymer-based (TransIT-X2), and lipopolyplex-based (TransIT-PRO).
Lipofectamine RNAiMax (Thermo) Opti-MEM (Thermo) siPool reverse transfection protocol for adherent cells with 1 or 3 nM final siRNA concentration (for amounts and volumes see Tables 1 and 2).
plasmids was carried out using Lipofectamine 3000 Reagents (Introgen) according to the protocol of manufacturer. To establish stably infected cell lines with CD63 overexpression or knockdown, RNA inference lenti-virus (sequence of siCD63 was used) and CD63 overexpression lentivirus were purchased from GenePharma, Shanghai China. Cells were infected
COS-7 cells were transfected with plasmid constructs as indicated with Lipofectamine 2000 according to the manufacturer’s protocol 24 to 48 hours before imaging. Cells were stained with SiR-Lysosome at 1 μM 4 hours before imaging. Cells were imaged in a microscope stage top micro-incubator (OKO Lab) with continuous air supply (37°C and 5% ...
Boost Lipofectamine2000 transfection efficiency with reduced cell toxicity. MagnetoFectamine short protocol. Magnetofection Brochure magnetic-assisted transfection, NeuroMag, CombiMag, LipoMag...
For electrophysiological analysis, CRACM1 protein was overexpressed in HEK293 cells stably expressing STIM2 protein (Soboloff et al. 2006) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) and the GFP expressing cells were selected by fluorescence. Experiments were performed 24–72 h after transfection.